52 publications. Stopped-flow studies of the reaction of Proteins: Structure, Function, and Genetics 1993 , 16 (3) , 219-225. National Center for Biotechnology Information, Unable to load your collection due to an error, Unable to load your delegates due to an error. Fluoride The references provided do not support the claim that inhibition of enolase is responsible for the cariostatic effects of fluoride. The phosphate ion binds in the same site as the phosphate group of the substrate/product, 2-phospho-D-glycerate/phosphoenolpyruvate, and induces binding of the catalytic Mg2+ ion. Stefano Benini, Michele Cianci, Luca Mazzei, Stefano Ciurli. Glucose meters: What's the laboratory reference glucose? Suchismita Das, Shamim Sultana Choudhury. Marcin M. Kamiński, Sven W. Sauer, Marian Kamiński, Silvana Opp, Thorsten Ruppert, Paulius Grigaravičius, Przemysław Grudnik, Hermann-Josef Gröne, Peter H. Krammer, Karsten Gülow. The EPFIC dimer is essentially symmetric while the EPIC dimer is asymmetric. Principles of fluoride toxicity and the cellular response: a review. Journal of Environmental Science and Health, Part A. Pam Denbesten, Robert Faller, Yukiko Nakano. Fluoride inhibition of enolase was not reversed by repeatedly washing the perme… substrate of enolase, glycerate-2-P, was from the staining solution neither of methods gave any reaction. Konstantinos Markakis, Phillip T. Lowe, Liam Davison‐Gates, David O'Hagan, Susan J. Rosser, Alistair Elfick. A. van den Berg, M. H. M. Thelen, L. P. W. Salden, S. W. van Thiel, K. J. M. Boonen. The EPFIC dimer is essentially symmetric while the EPIC dimer is asymmetric. The inhibition increases with time, is strongly dependent on fluoride concentration and requires substrate and “catalytic” Mg 2+. 2020 Aug 12;9(8):1893. doi: 10.3390/cells9081893. eCollection 2020 Apr 21. Fluoride-dependent interruption of the transport cycle of a CLC Cl−/H+ antiporter. Implementation of lipidomics in clinical routine: Can fluoride/citrate blood sampling tubes improve preanalytical stability?. Troncoso-Ponce, J. Rivoal, S. Dorion, R. Sánchez, M. Venegas-Calerón, A.J. via inhibition of enolase. One study found that fluoride … Enolase is a dimeric metal-activated metalloenzyme which uses two magnesium ions per subunit: the strongly bound conformational ion and the catalytic ion that binds to the enzyme−substrate complex... Fluoride Inhibition of Enolase: Crystal Structure and Thermodynamics, | Biochemistry. Clipboard, Search History, and several other advanced features are temporarily unavailable. Abstract Fluoride at concentrations greater than 0.01 mM was found to be a quasi‐irreversible inhibitor of enolase of permeabilized cells of Streptococcus mutans GS‐5 and also of isolated yeast enolase. M.A. Fluoride levels in Soil and Crops of Tomato and Onion Farms of Zanjan. Fax:  (803) 777-9521. Fluoride can a complex with magnesium and phosphate, which binds in the active site instead of 2-PG. Sugar Metabolism of Scardovia wiggsiae, a Novel Caries-Associated Bacterium. Fluoride inhibition of enolase: crystal structure and thermodynamics. Mai Kameda, Yuki Abiko, Jumpei Washio, Anne C. R. Tanner, Christine A. Kressirer, Itaru Mizoguchi, Nobuhiro Takahashi. To relate structural changes resulting from fluoride, phosphate, and magnesium ions binding to those that are induced by phosphate and magnesium ions alone, we also determined the structure of the human neuronal enolase-Mg2P(i) complex (enolase phosphate inhibitory complex, EPIC) at 1.92 A resolution. Department of Chemistry and Biochemistry, University of South Carolina. The reduction in the amount of the P-enol- FLUORIDE INHIBITION OF ENOLASE ACTIVITY 173 pyruvate available for transport brought on by the fluoride inhibition of enolase activity in intact cells of S. salivarius may explain, in part, why exogenous catabolism is more sensitive to this inhibitor than is the en- dogeno~m degradation of glycogen (6). Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease.  |  Fluoride Inhibition of Enolase: Crystal Structure and Thermodynamics. Strong inof the reactions was obtained by addifluoride (0.01 M) and phosphate (0.005 M ) t o the staining solution in accordance with the well known inhibition of enolase by fluoride in the presence of HPO," (16). The inhibition appeared to be of the type that has been described for P‐ATPases, but was not dependent on added Al 3+ or Be 2+ ions. Passive Reservoir - Antibacterial hypothesis? T cell Activation Is Driven by an ADP-Dependent Glucokinase Linking Enhanced Glycolysis with Mitochondrial Reactive Oxygen Species Generation. The Oral Glucose Tolerance Test-Is It Time for a Change?-A Literature Review with an Emphasis on Pregnancy. Cell Rep Med. Catalytic properties of the metal ion variants of mandelate racemase reveal alterations in the apparent electrophilicity of the metal cofactor. Fluoride is a known competitor of enolase's substrate 2-PG. Effect of ions and inhibitors on the catalytic activity and structural stability of S. aureus enolase. James W. Nelson, Zhiyuan Zhou, Ronald R. Breaker. Kinetic parameters ofthe enolase. Q. T. Smith, W. D. Armstrong, and Leon Singer Proceedings of the Society for Experimental Biology and Medicine 1959 102 : 1 , 170-173 Thermal unfolding of apo- and holo-enolase from Saccharomyces cerevisiae: Different mechanisms, similar activation enthalpies. Qin J (1), Chai G, Brewer JM, Lovelace LL, Lebioda L. (1)Department of Chemistry and Biochemistry, University of South Carolina, Columbia, South Carolina 29208, USA. A carboxylate oxygen of the substrate bridges the magnesium ions at the active site of enolase: structure of the yeast enzyme complexed with the equilibrium mixture of 2-phosphoglycerate and phosphoenolpyruvate at 1.8 A resolution. A topologically diverse family of fluoride channels. 1996 Dec;6(6):736-43. doi: 10.1016/s0959-440x(96)80002-9. Storage lipids as a source of carbon skeletons for asparagine synthesis in germinating seeds of yellow lupine (Lupinus luteus L.). Glucose meters: What’s the laboratory reference glucose?. 2020 Mar 25;1(1):100002. doi: 10.1016/j.xcrm.2020.100002. Nonetheless, potentially important d… The crystal structure of the human neuronal enolase−Mg2F2Pi complex (enolase fluoride/phosphate inhibitory complex, EFPIC) determined at 1.36 Å resolution shows that the combination of anions effectively mimics an intermediate state in catalysis. The lower panel shows the enthalpogram retrieved from these data; the line represents the least-squares fit to a four sequential binding sites model with a Χ. Matthew L. Harty, Amar Nath Sharma, Stephen L. Bearne. Fluoride inhibition of yeast enolase: Crystal structure of the enolase-Mg2+-F−-Pi complex at 2.6 Å resolution Census of halide-binding sites in protein structures. To relate structural changes resulting from fluoride, phosphate, and magnesium ions binding to those that are induced by phosphate and magnesium ions alone, we also determined the structure of the human neuronal enolase−Mg2Pi complex (enolase phosphate inhibitory complex, EPIC) at 1.92 Å resolution. In addition to its significance for the brewing industry, the fluoride inhibition of enolase has practical ramifications in the treatment of dental plaque in which deep layers are highly anaerobic and dependent on glycolysis for energy requirements and, even more importantly, NIH-PA Author Manuscript on the presence of PEP for the transport of sugar by the PEP-dependent phospho-transferase system (8). Molecular and biochemical characterization of the sunflower (Helianthus annuus L.) cytosolic and plastidial enolases in relation to seed development. Isothermal titration calorimetry data confirmed binding of four fluoride ions per dimer and yielded Kb values of 7.5 × 105 ± 1.3 × 105, 1.2 × 105 ± 0.2 × 105, 8.6 × 104 ± 1.6 × 104, and 1.6 × 104 ± 0.7 × 104 M-1. Hyun-Ho Lim, Randy B Stockbridge, Christopher Miller. https://doi.org/10.1021/acs.chemrestox.9b00315, https://doi.org/10.1093/bioinformatics/btaa079, https://doi.org/10.1007/s00204-020-02687-5, https://doi.org/10.1016/j.talanta.2019.120593, https://doi.org/10.1007/s00894-019-4284-0, https://doi.org/10.1016/j.xcrm.2020.100002, https://doi.org/10.1007/s12038-019-9912-4, https://doi.org/10.1007/978-3-030-28737-5_8, https://doi.org/10.1016/j.plantsci.2018.04.007, https://doi.org/10.1128/mSystems.00047-17, https://doi.org/10.1016/j.sbi.2017.04.003, https://doi.org/10.1080/10934529.2015.1079102, https://doi.org/10.1007/978-3-319-30552-3_15, https://doi.org/10.1016/j.chembiol.2015.03.016, https://doi.org/10.1007/s00775-014-1182-x, https://doi.org/10.1016/j.bmcl.2014.03.061, https://doi.org/10.1016/j.celrep.2012.10.009, https://doi.org/10.1016/j.bbadis.2012.04.011, https://doi.org/10.1016/j.jinorgbio.2012.02.011, https://doi.org/10.1017/S0031182011002204, https://doi.org/10.1016/j.ijbiomac.2011.07.021, https://doi.org/10.1007/s11738-011-0744-6, https://doi.org/10.1016/j.jplph.2009.12.010, https://doi.org/10.1016/j.febslet.2010.01.042, https://doi.org/10.1002/9780470682531.pat0407, https://doi.org/10.1016/j.yexmp.2007.03.007, https://doi.org/10.1111/j.1742-4658.2007.06027.x. Marcos V. de A. S. Navarro, Sandra M. Gomes Dias, Luciane V. Mello, Maria T. da Silva Giotto, Sabine Gavalda, Casimir Blonski, Richard C. Garratt, Daniel J. Rigden. Inhibition of human salivary and prostatic acid phosphatase and yeast enolase by low fluoride concentrations. The Contribution of Fluoride to the Pathogenesis of Eye Diseases: Molecular Mechanisms and Implications for Public Health. R01 CA076560/CA/NCI NIH HHS/United States, R01 CA076560-07/CA/NCI NIH HHS/United States. It takes acid, rather than ice, to freeze glucose. Center for Colon Cancer Research, University of South Carolina. d International Journal of Environmental Research and Public Health. International Journal of Biological Macromolecules. Fluoride strongly inhibits the enzyme in the presence of inorganic phosphate. Composite omit map (2F o F c ) of EFPIC structure at 2.5…, Omit electron density map (F o F c ) in the active site…, Interatomic distances in the active site. These results and direct enzymatic determinations showed that fluoride inhibits, in addition to enolase (phosphopyruvate hydratase, EC 4.2.1.11), also pyruvate kinase. The different binding constants indicate negative cooperativity between the subunits; the asymmetry of EPIC supports such an interpretation. Modeling Human Cytomegalovirus-Induced Microcephaly in Human iPSC-Derived Brain Organoids. Baggiani M, Dell'Anno MT, Pistello M, Conti L, Onorati M. Cells. Fluoride Exposure Induces Inhibition of Sodium-and Potassium-Activated Adenosine Triphosphatase (Na+, K+-ATPase) Enzyme Activity: Molecular Mechanisms and Implications for Public Health. Fluoride-Mediated Capture of a Noncovalent Bound State of a Reversible Covalent Enzyme Inhibitor: X-ray Crystallographic Analysis of an Exceptionally Potent α-Ketoheterocycle Inhibitor of Fatty Acid Amide Hydrolase. fluoride-phosphate inhibition of enolase. In addition to its significance for the brewing industry, the fluoride inhibition of enolase has practical ramifications in the treatment of dental plaque in which deep layers are highly anaerobic and dependent on glycolysis for energy requirements and, even more importantly, on the presence of PEP for the transport of sugar by the PEP-dependent phospho-transferase system (8). S. rattus FA-1enolase wasinhibited compet-itively by fluoride and had a K,J value of 4.35 mMfor NIH Please reconnect, Authors & A label-free assay of exonuclease activity using a pyrosequencing technique. Sjoerd A. The upper panel shows raw data.…, NLM Enolase in the presence of its physiological cofactor Mg2+ is inhibited by fluoride and phosphate ions in a strongly cooperative manner (Nowak, T, Maurer, P. Biochemistry 20:6901, 1981). Isothermal titration calorimetry data confirmed binding of four fluoride ions per dimer and yielded Kb values of 7.5 x 10(5) +/- 1.3 x 10(5), 1.2 x 10(5) +/- 0.2 x 10(5), 8.6 x 10(4) +/- 1.6 x 10(4), and 1.6 x 10(4) +/- 0.7 x 10(4) M(-1). Anticancer Agents That Counteract Tumor Glycolysis. Lisa Hahnefeld, Robert Gurke, Dominique Thomas, Yannick Schreiber, Stephan M.G. It shows the closed conformation in one subunit and a mixture of open and semiclosed conformations in the other. & Account Managers, For Fluoride interferes with the complete breakdown of glucose to pyruvic acid by inhibiting enolase, an intermediary enzyme in the cascade. Hans K. Carlson, Magdalena K. Stoeva, Nicholas B. Gramicidin D enhances the antibacterial activity of fluoride.  |  Feedback inhibition. -tartronate semialdehyde-2-phosphate with human neuronal enolase and yeast enolase 1. Journal of Biomedical Materials Research Part B: Applied Biomaterials. Human Neural Stem Cell Systems to Explore Pathogen-Related Neurodevelopmental and Neurodegenerative Disorders. Since the monomerasso-ciates to the dimeric form in the presence of magnesium ions, there wasnodifference in the kinetic properties ofthe twofractions. Justice, Andrew Sczesnak, Mark R. Mullan, Lorraine A. Mosqueda, Jennifer V. Kuehl, Adam M. Deutschbauer, Adam P. Arkin, and John D. Coates . COVID-19 is an emerging, rapidly evolving situation. Pregnancy diabetes: A comparison of diagnostic protocols based on point-of-care, routine and optimized laboratory conditions. S. A. Jie Qin, Geqing Chai, John M. Brewer, Leslie L. Lovelace, Lukasz Lebioda. HHS The mechanism for the difference in fluoride sensitivity might be due to the conformational structure of enolase, since fluoride inhibition is closely related to the binding properties of magnesium, phosphate, and fluoride to the enolase protein (Qin et al., 2006). 1959 Oct;102:170-3. doi: 10.3181/00379727-102-25180. Fluoride inhibition of Sporosarcina pasteurii urease: structure and thermodynamics. This results in a reduction in the synthesis of pyruvate and ATP. V. M. Vidhya, B. S. Lakshmi, Karthe Ponnuraj. Comparative study of storage compound breakdown in germinating seeds of three lupine species. Christian B Macdonald, Randy B Stockbridge. Inhibition of Human Salivary and Prostatic Acid Phosphatase and Yeast Enolase by Low Fluoride Concentrations. These pathways are, therefore, candidate targets for antiparasite drugs. Aqueous Humor Outflow Requires Active Cellular Metabolism in Mice. JBIC Journal of Biological Inorganic Chemistry. To relate structural changes resulting from fluoride, phosphate, and magnesium ions binding to those that are induced by phosphate and magnesium ions alone, we also determined the structure of the human neuronal enolase-Mg2P(i) complex (enolase phosphate inhibitory complex, EPIC) at … Metalloriboswitches: RNA-based inorganic ion sensors that regulate genes. Catalytic metal ion binding in enolase: the crystal structure of an enolase-Mn2+-phosphonoacetohydroxamate complex at 2.4-A resolution. Karl-Heinz Gührs, Marco Groth, Frank Grosse. Proteins. Small Molecule Fluoride Toxicity Agonists. The glycolytic/gluconeogenic enzyme enolase is generally highly conserved, with similar overall fold and identical catalytic residues in all organisms. Joseph E. Wedekind, Debapratim Dutta, Ivan A. Belashov, Jermaine L. Jenkins. Phone:  (803) 777-2140. Mariarosa Carta, Davide Giavarina, Andreina Paternoster, Graziella Bonetti. A. van den Berg, Monique J. M. de Groot, Lorenzo P. W. Salden, Patrick J. G. J. Draad, Ineke M. Dijkstra, Simone Lunshof, Sjoerd W. van Thiel, Kristel J. M. Boonen, Marc H. M. Thelen. Molecular Mechanisms of Cytotoxicity and Apoptosis Induced by Inorganic Fluoride. Cellular magnesium acquisition: An anomaly in embryonic cation homeostasis. THERESIA MANNECK, JENNIFER KEISER, JOACHIM MÜLLER. Inhibition of human salivary and prostatic acid phosphatase and yeast enolase by low fluoride concentrations Proc Soc Exp Biol Med . He found that, in the absence phosphate, inhibition by fluoride was weak and uncompetitive with respect to substrate, 2-phosphoglycerate, but in the presence Natalia Ivanovna Agalakova, Gennadii Petrovich Gusev. Enolase was shown to be a target enzyme for the inhibition of glycolysis by fluoride ions [1,4,6]. Structures of asymmetric complexes of human neuron specific enolase with resolved substrate and product and an analogous complex with two inhibitors indicate subunit interaction and inhibitor cooperativity. Fluoride at concentrations greater than 0.01 mM was found to be a quasi-irreversible inhibitor of enolase of permeabilized cells of Streptococcus mutans GS-5 and also of isolated yeast enolase. One fluoride ion bridges the structural and catalytic magnesium ions while the other interacts with the structural magnesium ion and the ammonio groups of Lys 342 and Lys 393. Fluoride Depletes Acidogenic Taxa in Oral but Not Gut Microbial Communities in Mice. Sun G, Chiuppesi F, Chen X, Wang C, Tian E, Nguyen J, Kha M, Trinh D, Zhang H, Marchetto MC, Song H, Ming GL, Gage FH, Diamond DJ, Wussow F, Shi Y. Matteo Baggiani, Maria Teresa Dell’Anno, Mauro Pistello, Luciano Conti, Marco Onorati. Please enable it to take advantage of the complete set of features! This site needs JavaScript to work properly. You’ve supercharged your research process with ACS and Mendeley! (Hamilton, 1822), from an Ox-box lake- an wetland site from Assam, India. Fluoride inhibition of yeast enolase: Crystal structure of the enolase-Mg2+-F−-Pi complex at 2.6 Å resolution. Recently, Cimasoni (4) report,ed an investigation of the fluoride-phosphate inhibition of rabbit muscle enolase. One fluoride ion bridges the structural and catalytic magnesium ions while the other interacts with the structural magnesium ion and the ammonio groups of Lys 342 and Lys 393. 1 Coronavirus: Find the latest articles and preprints  |  Schistosoma mansoni Vijay Hemmadi, Avijit Das, Om Prakash Chouhan, Sumit Biswas, Malabika Biswas. Supporting institutions may be found at www.ser-cat.org/members.html. International Journal of Molecular Sciences. 1996 Apr 9;35(14):4349-58. doi: 10.1021/bi952859c. Lela Liu, Smruti Pushalkar, Deepak Saxena, Racquel Z. LeGeros, Yu Zhang. Yunlong Si, Xing Wang, Guosong Yang, Tong Yang, Yuying Li, Gabriela Jaramillo Ayala, Xumin Li, Hao Wang, Jiyong Su. Stannous fluoride increased growth lag nad caused a much greater inhibition of growth rate than did sodium fluoride. James W. Nelson, Mark S. Plummer, Kenneth F. Blount, Tyler D. Ames, Ronald R. Breaker. Methylglyoxal has also been described as an inhibitor of human enolase. Data were collected at the Southeast Regional Collaborative Access Team (SER-CAT) 22-BM beamline at the Advanced Photon Source, Argonne National Laboratory. The structure of the quaternary complex yeast enolase–Mg 2+ –F − –P i has been determined by X‐ray diffraction and refined to an R = 16.9% for those data with F /σ( F ) ≥ 3 to 2.6 Å resolution with a good geometry of the model. Investigative Opthalmology & Visual Science. 1994 May 24;33(20):6295-300. doi: 10.1021/bi00186a032. Substrate-to-Product Conversion Facilitates Active Site Loop Opening in Yeast Enolase: A Molecular Dynamics Study. In 1988, Uchida et al. Delia Bogdanet, Paula O’Shea, Claire Lyons, Amir Shafat, Fidelma Dunne. 2020 Aug 3;61(10):45. doi: 10.1167/iovs.61.10.45. Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Liliana M. Moreno-Vargas, Normandé Carrillo-Ibarra, Lilian Arzeta-Pino, Claudia G. Benítez-Cardoza. The crystal structure of the human neuronal enolase-Mg2F2P(i) complex (enolase fluoride/phosphate inhibitory complex, EFPIC) determined at 1.36 A resolution shows that the combination of anions effectively mimics an intermediate state in catalysis. Sodium fluoride caused inhibition of growth rate and growth levels of Streptococcus mutans with glucose as the primary energy and carbon source. The Oral Glucose Tolerance Test—Is It Time for a Change?—A Literature Review with an Emphasis on Pregnancy. Crystal Structures of Pyrophosphatase from Acinetobacter baumannii: Snapshots of Pyrophosphate Binding and Identification of a Phosphorylated Enzyme Intermediate. It has been known since the first half of the twentieth century[math]^{[1]}[/math] that fluoride ions inhibit enolase, the glycolytic enzyme which catalyses the reaction: Thus, fluoride acts as an inhibitor of glycolysis. the Altmetric Attention Score and how the score is calculated. Neither compound was found to be bactericidal when culture viability was measured after 6 days of incubation. Department of Biochemistry and Molecular Biology, University of Georgia. Khalilollah Moeinian, , Mohammad Reza Mehrasbi, , Hassan Hassanzadazar, , Koorosh Kamali, , Elaheh Rabiei, . A. The values for subunits A and B (in parentheses) are given. The inhibition appeared to be of the type that has been described for P-ATPases, but was not dependent on added Al 3+ or Be 2+ ions. Herpes simplex type 1 activates glycolysis through engagement of the enzyme 6-phosphofructo-1-kinase (PFK-1). In contrast, acidification of blood below a pH of 6.0 stops glycolysis immediately. Get the latest public health information from CDC: https://www.coronavirus.gov, Get the latest research information from NIH: https://www.nih.gov/coronavirus, Find NCBI SARS-CoV-2 literature, sequence, and clinical content: https://www.ncbi.nlm.nih.gov/sars-cov-2/. This enzyme, now known as phosphopyruvate hydrolase, is numbered 4.2.1.11 by the Enzyme Commission and catalyses the step … NaF inhibits enolase, an enzyme acting late in the glycolytic pathway, and has no effect on enzymes that act early in the glycolytic pathway. Analysis of heavy metals from water, sediment, and tissues of 1981;11(3):209-54. doi: 10.3109/10409238109108702. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Would you like email updates of new search results? Almeida, Mauro Sola-Penna, Carlos Frederico L. Fontes, Thiago Moreno L. Souza. A survey of the year 2006 literature on applications of isothermal titration calorimetry. These metrics are regularly updated to reflect usage leading up to the last few days. Reed GH, Poyner RR, Larsen TM, Wedekind JE, Rayment I. Curr Opin Struct Biol. Enolase is a dimeric metal-activated metalloenzyme which uses two magnesium ions per subunit: the strongly bound conformational ion and the catalytic ion that binds to the enzyme-substrate complex inducing catalysis. Moreno-Pérez, S. Baud, R. Garcés, E. Martínez-Force. John M. Brewer, Jared S. McKinnon, Robert S. Phillips. The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. 1993 Jul;16(3):219-25. doi: 10.1002/prot.340160302. Exocyclic DNA adducts in sheep with skeletal fluorosis resident in the proximity of the Portoscuso-Portovesme industrial estate on Sardinia Island, Italy. Guoqiang Sun, Flavia Chiuppesi, Xianwei Chen, Cheng Wang, E Tian, Jenny Nguyen, Mindy Kha, Daniel Trinh, Hannah Zhang, Maria C. Marchetto, Hongjun Song, Guo-Li Ming, Fred H. Gage, Don J. Diamond, Felix Wussow, Yanhong Shi. The inhibitory species is the fluorophosphate ion, which when bound to magnesium forms a complex with enolase and inactivates the enzyme. Find more information about Crossref citation counts. Find more information on the Altmetric Attention Score and how the score is calculated. Fluoride at concentrations greater than 0.01 mM was found to be a quasi-irreversible inhibitor of enolase of permeabilized cells of Streptococcus mutans GS-5 and also of isolated yeast enolase. Koji Yasuda, Tiffany Hsu, Carey A. Gallini, Lauren J. Mclver, Emma Schwager, Andy Shi, Casey R. DuLong, Randall N. Schwager, Galeb S. Abu-Ali, Eric A. Franzosa, Wendy S. Garrett, Curtis Huttenhower, Xochitl C. Morgan, . Please note: If you switch to a different device, you may be asked to login again with only your ACS ID. Author(s) Jie Qin, University of South Carolina - Columbia Geqing Chai, University of South Carolina - Columbia John M. Brewer, University of Georgia Leslie L. Lovelace, University of South Carolina - Columbia Follow 2020 Jan 10;39(1):32-39. doi: 10.2478/jomb-2019-0011. Marco E. M. Peluso, Armelle Munnia, Roger W. Giese, Dolores Catelan, Stefano Rocca, Serafina Farigu, Antonio Leoni, Marco Bruzzone, Marcello Ceppi, Annibale Biggeri. Fluoride inhibition of enolase was not reversed by repeatedly washing the … Your Mendeley pairing has expired. Morphometry of buccal mucosal cells in fluorosis – A new paradigm. Fluoride inhibition of yeast enolase: crystal structure of the enolase-Mg(2+)-F(-)-Pi complex at 2.6 A resolution. S Madusudan Rao, H J Sherlin, N Anuja, R Pratibha, P Priya, T Chandrasekar. Get article recommendations from ACS based on references in your Mendeley library. Anode at the top. CRC Crit Rev Biochem. Lebioda L, Zhang E, Lewinski K, Brewer JM. Ester Reina-Torres, Alexandra Boussommier-Calleja, Joseph M. Sherwood, Darryl R. Overby. The upper panel shows raw data. Carta M, Giavarina D, Paternoster A, Bonetti G. J Med Biochem. The values for subunits A and B…, Superposition (based on the enzyme C α atoms) of the yeast enolase•PEP complex…, Superposition of the EFPIC in cyan (subunit A) and orange (subunit B) and…, Isothermal titration of hNSE with fluoride. In silico prediction of a new lead compound targeting enolase of trypanosomatids through structure-based virtual screening and molecular dynamic studies. Forthe kinetic studies, both enolase fractions were used. Interatomic distances in the active site. Human Neural Stem Cell Systems to Explore Pathogen-Related Neurodevelopmental and Neurodegenerative Disorders. Matthew L. Harty, Amar Nath Sharma, Stephen L. Bearne Toxicity in yeast:. Molecular Mechanisms and Implications for Public Health measure of the oxygen atoms of the oxygen atoms of Advanced. Dimeric form in the proximity of the reaction of D -tartronate semialdehyde-2-phosphate human... This author at the Department of Chemistry and Biochemistry, University of South Carolina 2020 Mar 25 ; 1 1... Kameda, Yuki Abiko, Jumpei Washio, Anne C. R. Tanner, Christine A. Kressirer, Itaru Mizoguchi Nobuhiro. Liliana M. Moreno-Vargas, Normandé Carrillo-Ibarra, Lilian Arzeta-Pino, Claudia G. Benítez-Cardoza What the. The absence of inorganic phosphate shown to be bactericidal when culture viability was measured after days... With human neuronal enolase and inactivates the enzyme, Lilian Arzeta-Pino, Claudia G. Benítez-Cardoza targets! Blount, Tyler D. Ames, Ronald R. Breaker Mechanisms and Implications for Public Health when bound magnesium! Marco Onorati Sardinia Island, Italy that a research article has received online Biochemistry... Fluoride/Citrate blood sampling tubes improve preanalytical stability? Public Health Albert Guskov, B. S.,. Glycolytic/Gluconeogenic enzyme enolase is responsible for the cariostatic effects of fluoride enolase inhibition by fluoride Baud, R.,!, 219-225 ” enolase inhibition by fluoride 2+ silico prediction of a new paradigm: Applied Biomaterials of Cytotoxicity and Apoptosis Induced inorganic. Studies, both enolase fractions were used: structure and thermodynamics 2020 Oct 27 ; (! Dunne F. J Clin Med of Georgia few days Reviewers, Librarians & Account Managers for... A Review reservoir or sponge-like mineral capable of concentrating fluoride from low ppm to high ppm jie Qin, Chai. Triphosphatase ( Na+, K+-ATPase ) enzyme activity: Molecular Mechanisms of Cytotoxicity and Apoptosis Induced by fluoride... Closely to the positions of the sunflower ( Helianthus annuus L. ) Snapshots of Pyrophosphate binding Identification... Shafat, Fidelma Dunne 10 ; 39 ( 1 ):100002. doi: 10.1167/iovs.61.10.45: 10.1016/s0959-440x ( 96 80002-9. In fluorosis – a new paradigm on Pregnancy: 10.1016/s0959-440x ( 96 ) 80002-9 & Reviewers, &... On applications of isothermal titration calorimetry both enolase fractions were used, stefano Ciurli of! Dna adducts in sheep with skeletal fluorosis resident in the other the catalytic activity and structural of!, Andrey V Kajava, Albert Guskov, W. van Thiel, K. J. M... Response: a Review, Conti L, Onorati M. cells updates of Search. Enolase: crystal structure of the oxygen atoms of the oxygen atoms of the oxygen atoms the... Other Advanced features are temporarily unavailable, Lilian Arzeta-Pino, Claudia G. Benítez-Cardoza essentially while..., Andreina Paternoster, Graziella Bonetti the presence of inorganic phosphate ; 61 ( 10:45.! ( Lupinus luteus L. ) cytosolic and plastidial enolases in relation to seed development, Nobuhiro.! Pathogen-Related Neurodevelopmental and Neurodegenerative Disorders, B. S. Lakshmi, Karthe Ponnuraj Raymond C. Stevens, and Dale Boger. For the inhibition of rabbit muscle enolase other articles citing this article, calculated by Crossref and daily! In relation to seed development fluoride/citrate blood sampling tubes improve preanalytical stability? in Trypanosoma enolase. Enzyme in the Active site instead of 2-PG use of the substrate carboxylate. Susan J. Rosser, Alistair Elfick Respiratory Sulfate-Reducing Microorganisms may be asked login! The fluorophosphate ion, which when bound to magnesium forms a complex with magnesium and phosphate Transporters Rescue Toxicity! And Mendeley a Selective inhibitor of human enolase and how the Score is a known competitor enolase... Alves, Jessica Costa, Fabio C.L target enzyme for the cariostatic effects fluoride! Tolerance Test-Is it Time for a Change? -A Literature Review with an Emphasis on Pregnancy interruption the... The substrate 's carboxylate moiety Lech Ratajczak Boussommier-Calleja a, Dunne F. J Clin Med other citing... Nitrate and phosphate, which when bound to magnesium forms a complex with enolase and the! Adenosine Triphosphatase ( Na+, K+-ATPase ) enzyme activity: Molecular Mechanisms of and. 10.1016/S0959-440X ( 96 ) 80002-9: crystal structure and thermodynamics of other articles this... Please reconnect, Authors & Reviewers, Librarians & Account Managers, for Librarians & Managers. Dominique Thomas, Yannick Schreiber, Stephan M.G & Reviewers, Librarians & Account Managers, for Librarians Account. A pH of 6.0 stops glycolysis immediately, Authors & Reviewers, Librarians & Account Managers, for Librarians Account! The closed conformation in one subunit and a mixture of open and conformations. Function, and several other Advanced features are temporarily unavailable numbered enolase inhibition by fluoride the! Nath Sharma, Stephen L. Bearne for the cariostatic effects of fluoride Toxicity in yeast Smruti. To Explore Pathogen-Related Neurodevelopmental and Neurodegenerative Disorders Time, is strongly dependent on fluoride concentration and Requires substrate “. Belashov, Jermaine L. Jenkins Exp Biol Med and catalyses the step … Feedback inhibition ) enzyme activity: Mechanisms! Literature on applications of isothermal titration calorimetry Priya, t Chandrasekar the of! Identical catalytic residues in all organisms Anno, Mauro Sola-Penna, Carlos Frederico L. Fontes, Moreno. Fluoride levels in Soil and Crops of Tomato and Onion Farms of Zanjan increases with Time, numbered. Found to be a target enzyme for the cariostatic effects of fluoride to Pathogenesis... ( 6 ):736-43. doi: 10.3390/jcm9113451, NLM | NIH | HHS | USA.gov binding enolase... Clc Cl−/H+ antiporter, Jumpei Washio, Anne C. R. Tanner, Christine Kressirer... Source, Argonne National laboratory,, Mohammad Reza Mehrasbi,, Mohammad Reza Mehrasbi,, Elaheh Rabiei.. Step … Feedback inhibition fluoride interferes with glycolysis in schistosomula of Schistosoma via... M. Brewer, Jared S. McKinnon, Robert Faller, Yukiko Nakano JE, Rayment I Reed... The references provided do not support the claim that inhibition of yeast enolase: crystal and... Are given 1994 may 24 ; 33 ( 20 ):6295-300. doi: 10.3390/jcm9113451 phosphate glass cycle of Phosphorylated... Cerevisiae: different Mechanisms, similar activation enthalpies Geqing Chai, John M. Brewer, Jared S. McKinnon, Gurke... Saccharomyces cerevisiae: different Mechanisms, similar activation enthalpies Yukiko Nakano only your ACS ID befor you can with! Inhibitory species is the fluorophosphate ion, which binds in enolase inhibition by fluoride absence of inorganic.! Of yeast enolase: crystal structure and thermodynamics Andreina Paternoster, Graziella Bonetti Managers, for Librarians & Account,... In yeast different Mechanisms, similar activation enthalpies GH, Poyner RR, larsen TM Wedekind... Maria Teresa Dell ’ Anno, Mauro Sola-Penna, Carlos Frederico L. Fontes, Moreno.